Abstract
Phytoplasma is a cell wall-less bacteria that obligate parasites in phloem and causes virescence and phyllody on flowers. In this study, we confirm the peanut witches’ broom (PnWB) phytoplasma infection in Catharanthus roseus and first report the morphology and size of PnWB phytoplasma. In addition, the PHYL1Pn, an effector of PnWB phytoplasma, causes virescence and phyllody phenotypes in Arabidopsis (herein leafy flower), which similar to SHORT VEGATATIVE PHASE (SVP) over-expression plants. In PHYL1Pn plants, the miR396 was down-regulated whereas the AtSVP was up-regulated and the leaf and root phenotypes of the transgenic plants were agreed with miR396 and AtSVP expression patterns. We suggested that PHYL1Pn may induce leafy flower transition through interfering miR396 and AtSVP expression. In this study, we built up an intergraded omics pipeline for a non-model organism research. The three high-throughput omics was used to verify the transcriptome and predicted miRNA targets profiles of C. roseus, and also used to identify novel miRNA targets in growth, development and metabolism related genes in C. roseus. The experimental methods including transgenic and mutant lines, northern blot and in situ hybridization were applied in confirming phytoplasma effector affecting miRNA/target regulation.
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