Abstract
For the selection of peptide specifically binding to Pb2+, the biopanning with the commercially available Ph.D.-7 phage displayed heptapeptide library was carried out against Pb2+ immobilized on a metal-chelating IDA (iminodiacetic acid) resin. After four rounds of screening against Pb 2+ -IDA including negative selections against charged bead with metal ions other than Pb2+ and uncharged bead, several candidate leadbinding phage peptides were initially determined based on the order of frequency from the screened phage clones. Of the selected phage peptide sequences, the peptide of the highest frequency, CysSerIleArgThrLeuHisGlnCys (CSIRTLHQC) also exhibited the strongest affinity for Pb 2+ in binding assays for individual phage clones. However, there was not a significant difference in Pb2+ affinity between selected peptides when using synthetic heptapeptides corresponding to the displayed peptide sequences of phage clones.
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