副乾酪乳酸桿菌 NTU 101 所生成降血壓物質之純化與大家優藻褐藻服可醫定膠囊調節血脂之功效評估

Abstract

Part 1 Soy is a traditional Chinese food, its protein contains a complete essential amino acids as an equivalent of animal protein. Many studies have also pointed out that soy and soy products have many benefits on human health, including the prevention of cardiovascular disease, kidney disease, diabetes, malignancy, obesity, osteoporosis, allergies, constipation, etc and improved health condition. The purpose of this study is using Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) to optimize the production conditions of angiotensin-converting enzyme (ACE) inhibition value with Response Surface Methodology (RSM). After separating and purifing active ingredients, nuclear magnetic resonance (NMR) was applied to identify isolated bioactive compounds. Among 16 groups measured, fermented soil milk extraction had the highest ACEI activity, with half-maximal inhibitory concentration (IC50) reduced from 1.22 mg/mL to 1.02 mg/mL under the follow condition: 12% soy milk, starting from pH 9.0 and 15% inoculum. NTU 101 soil milk alcohol extraction were separated and purified by different polarity organic liquids, Silica gel column chromatography, Sephadex LH20 column chromatography, TLC and HPLC. After that, purified compounds F1-6-2 had the most inhibitory activity. Purified compounds F1-6-2 was identified as Daidzein by NMR and FTIR. IUPAC name is 7-Hydroxy-3- (4-hydroxyphenyl) chromen-4-one. In conclusion, soy milk fermented by NTU 101 can produce bioactivity substances that inhibited ACE activity. In the future, to enhance the value of traditional soy milk products, this multifunction component can be applied to the development of functional foods to prevent the development of hypertension and associated diseases. Part 2 Many studies have pointed out a higher total cholesterol (TC) value in serum contributed to a higher death rate from coronary heart disease, and the percentage of saturated fat accounted for total calories in dietary showed a strong positive correlation with coronary heart disease (correlation coefficient r = 0.84). Excessive intake of saturated fatty acids in food will increase blood TC and low-density lipoprotein cholesterol (LDL-C), and promote atherosclerosis. And there are many studies indicating that abnormal blood lipids and high cholesterol diet are both important risk factors of cardiovascular diseases. The objective of this study was to determine the effect of fucoidan on lipid modulation of hypercholesterolemic hamsters fed with high cholesterol diet, evaluation for TC, triglycerides (TG), LDL-C, high-density lipoprotein cholesterol (HDL-C) and the antioxidant ability of blood, feces and liver. The results indicated that TC ,TG, the ratio of LDL-C to HDL-C of serum, and total liver TC and TG were significant decreased after feeding with fucoidan, compared with high cholesterol group. In addition, the level of the activities of superoxide dismutase and catalase were significantly increased in the fucoidan groups (p < 0.05), as compared to those in the high cholesterol group. In conclusion, fucoidan can regulate the blood lipid of hamsters fed with high cholesterol diet.