Функціональний стан печінки курчат-бройлерів за застосування мультиферментного препарату «Natuzyme»

Abstract

The results of investigation using multienzyme preparation «Natyzyme» for broiler chickens to provide ultimate digestion of feed by preventive treatment of digestive system organs, increase of poultry growth intensity and correction of physiological spectrum of homeostasis have been shown in this article. Fifty chickens of cross Ross 308 of first (experimental) group since the sixth day of growth were inserted preparation «Natyzyme» added to feed in a dose of 35 gr on 100 kg of feed during 30 days, while the poultry of second (control) group was not used that preparation. The influence of enzyme preparation «Natyzyme» was estimated on 14th and 36th day of poultry birth according to analysis of productive indices, the results of biochemical and histological researches. It was defined the chick livability and liveweight gain of poultry during the period of growth, feed conversion ratio and EEI (European efficiency index) of poultry operation. Blood sampling was carried out in above mentioned period to define indices, characterized functional liver state: protein contain, albumin, alanine transaminase, aspartate aminotransferase, cholinesterase, that were investigated spectrophotometrically. The liver samples were taken for histological research on the 36th day of poultry birth. Obtained digital data were processed mathematically of variation statics. The use of preparation «Natyzyme» contributed to broiler body weight gain on 5.1 % (р ≤ 0,05), viability – on 4.0 %. The results of biochemical researches of blood serum showed higher protein content in chickens of experimental group to 14 and 36th day of experience on 3.2 and 8.9 % (р ≤ 0,05) accordingly. In connection with increasing protein content the index of synthetic activity of liver has grown – cholinesterase contain: on 14th day of birth on 7.1 %, 36th day of birth – on 14.6 % (р ≤ 0,05). The indices of enzymes transamination activity - alanine transaminase, aspartate aminotransferase in blood serum of experimental chicken group on 14th and 36th day of birth accurately have not differed from control data. According to the histological analysis of liver the positive influence of multienzyme preparation on the chicken broilers body has been diagnosed. Unlike the chicken liver of control group, where the presence of liver beam decomplexation in the composition of particles and fatty degeneration of hepatocytes was detected, the chickens of experimental group did not have the such features of the microscopic structure. As a result, the use of preparation «Natyzyme» contributed to greater growth and safety of broiler chickens, reducing of feed costs per 1kg of poultry gain, increasing the European efficiency index of poultry, had a positive effect on the functional state of the liver, that blood biochemical results and data of histological researches have been proved, that shows the advantages of its use in the feeding of broiler chickens.