Экспрессия miR-181a и miR-146a при преждевременной овариальной недостаточности аутоиммунной этиологии

Abstract

Objective. To study the expression of miR-181a and miR-146a in the blood serum obtained from an experimental animal model of autoimmune oophoritis on different days of the experiment to assess their role in primary ovarian insufficiency (POI) of autoimmune etiology. Materials and methods. Experimental animals were mature female outbred rats. The study group (n = 25) included the experimental animal model of autoimmune oophoritis, the control group (n = 20) – intact animals. Morphometry of ovarian samples with estimating the average number of follicles, corpora lutea and atretic corpora lutea was performed. Enzyme immunoassay was used to study the concentration levels of serum anti-Mullerian Hormone (AMH) and anti-ovarian antibodies (AOA). MicroRNAs were isolated from serum according to a standard protocol; a reverse transcription and real-time polymerase chain reaction were performed using specific primers, and the relative expression of miR-181a and miR-146a was calculated by the Pfaffle method. Results. The expression of miR-146a on different days of the experiment remained statistically insignificant, including when compared to the control group. The expression of miR-181a in the blood serum of experimental animals on day 15 was statistically significantly higher than similar values on days 5, 10, 30 and 60 and in the control group. Conclusion. MiR-181a showed high diagnostic value in POI of autoimmune etiology in the experiment performed and can serve as an early marker in the diagnosis of ovarian insufficiency. The role of miR-146a as a marker of POI of autoimmune etiology has not been experimentally confirmed. Key words: autoimmune oophoritis, microRNA, experiment, premature ovarian insufficiency