Abstract

The genus Alfalfa of the legume family (Fabaceae) includes about 60 species of herbaceous plants. One of the most widespread representatives in Russia and Kazakhstan is Medicago lupulina L. In the scientific medicine of Russia and Kazakhstan Medicago lupulina L. is currently not used. There is no approved pharmacopoeial article for this plant. However, this species has long been used in traditional medicine for a variety of diseases. For introduction into scientific medicine, it is necessary to develop authenticity indicators and conduct a study of morphological and anatomical signs of the structure of Medicago lupulina L. flowers. Objective: to conduct a macro-and microscopic study of the structure of the alfalfa hop flowers; to identify and characterize the signs that are of differential importance in the diagnosis of raw materials. Materials and methods. The study of morphological and anatomical features of Medicago lupulina L. was carried out in accordance with the articles of the State Pharmacopoeia of the Russian Federation of the XIV edition and the State Pharmacopoeia of the Republic of Kazakhstan. Freshly harvested, dried and fixed raw materials were used for the study. Results. For the first time, macro- and microdiagnostic signs of flowers of Medicago lupulina L. were described. Conclusion. For the first time macroscopic and microscopic features of flowers of Medicago lupulina L. were identified and described, which can be used to determine the authenticity of raw materials, as well as to develop the sections «External signs» and «Microscopic signs» of modern regulatory documentation.

Highlights

  • Objective: to conduct a macro-and microscopic study of the structure of the alfalfa hop flowers; to identify and characterize the signs that are of differential importance in the diagnosis of raw materials

  • For the first time macroscopic and microscopic features of flowers of Medicago lupulina L. were identified and described, which can be used to determine the authenticity of raw materials, as well as to develop the sections «External signs» and «Microscopic signs» of modern regulatory documentation

  • For citation: Drozdova I.L., Trembalya Ya.S., Bogoslovskikh D.D., Orynbasarova K.K., Omiraliev M.A. Morphological and anatomical structure of Medicago Lupulina L. flowers

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Summary

МАТЕРИАЛЫ И МЕТОДЫ ИССЛЕДОВАНИЯ

Макро- и микроскопическое изучение строения люцерны хмелевидной цветков регламентировано действующей современной нормативной документацией: Государственной фармакопеей Российской Федерации XIV издания: ОФС.1.5.1.0004.15 «Цветки (Flores)» и ОФС.1.5.3.0003.15 «Техника микроскопического и микрохимического исследования лекарственного растительного сырья и лекарственных растительных препаратов» [5], а также Государственной фармакопеей Республики Казахстан: статья «Техника микроскопического и микрохимического исследования лекарственного растительного сырья» [4]. Использовали различное сырье люцерны хмелевидной цветков: цветки свежесобранные, высушенные (воздушно-теневая сушка), фиксированные в смеси (спирт этиловый 96% – глицерин – вода очищенная (1:1:1)) [7, 8, 20, 21]. Для получения микрофотографий исследуемых объектов применялась цифровая фотокамера. Для редактирования полученных изображений использована программа «Adobe Photoshop CC ×64».

РЕЗУЛЬТАТЫ ИССЛЕДОВАНИЯ И ИХ ОБСУЖДЕНИЕ
ЛИЧНЫЙ ВКЛАД АВТОРОВ
Materials and methods
Results
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