제주마의 기본모색과 MC1R과 ASIP 유전자형 조합의 상관관계

Abstract

This study was undertaken to reveal the relationship between genetic variations and the basic coat color classification system in Jeju horses. Genetic variations of the melanocortinreceptor 1 (MC1R) and agouti signaling protein (ASIP) genes were investigated using pyrosequencing technique. A nucleotide substitution mutation for MC1R g.901C>T and an ASIP 11-bp deletion mutation were screened. Black horses had MC1R E + /- (E + /E + or E + /E e ) and ASIP A a /A a genotypes. In contrast, chestnut horse genotypes were MC1R E e /E e and ASIP -/-. Thus, black and bay horses have at least one dominant MC1R allele, E + , whereas chestnut horses have homozygous recessive alleles E e /E e A allele represses black coat color development in the hairs of the body, but not in the mane and all four legs. Pedigree analysis showed a consistent relationship between the genotype distribution of the MC1R and ASIP genes and basic coat color patterns, even in the F1 progeny. The results of this study revealed the relationship between the coat color phenotype and genetic background and suggested that useful information may be provided for molecular breeding of Jeju horses. (Key words : Jeju horse, Coat color, Genetic variation, MC1R, ASIP)