Abstract
Thermostable mannanases have been identified from Thermotoga neapolitana , Rhodothermus marinus , Bacillus stearotherrnophilus , Thermo- anaerobacterium polysaccharolyticum , Caldocellosiruptor saccharolyticus ," and Dictyoglomus thermophilum . The β -mannanases from T. Neapolitana (family 5) and R. marinus (family 26) are the most thermostable of these. The mannanase from T. neapolitana is less stable at 85°, but more stable at 90°, than the corresponding enzyme from R. marinus . The former has a half-life of 34 hr at 85° and 13 hr at 90°, whereas the latter has a half life of 45.3 and 4.5 hr at the respective temperatures. Hyperthermophilic mannanases are useful in several industrial applications where thermostability and thermoactivity are desirable. These include coffee extraction, oil/gas well stimulation, and wood pulp treatment. This chapter describes the purification and characterization of a β -mannanase from T. neapolitana , as well as cloning and sequencing of this enzyme from T. neapolitana and T. maritima .
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