骨組織中の Lysozyme 活性

Abstract

The relationship between the lysozyme activity in bone tissues of the rat and the trace of behaviour of the enzyme activity in vivo has been investigated.The lysozyme activity in serum, mandibula, femur and femur marrow from each of 5 rats (strain of wister) was assayed by a modified method of Kakisaki.Also, the time-course of the changes of lysozyme activity in the serum, mandibula, femur and femur marrow from each of 75 rats was measured after the intra-peritoneal injection of lysozyme chloride (5, 10mg/100g) and 0.9% saline as a control.The tissues were removed immediately after the death of the rat which was slaughtered. The tissue samples were weighted, and then immediately homogenized at 7, 000rpm for 20sec. with cold M/15 phosphate buffer solution by using a cold polytron homogenizer. The homogenate was centrifuged at 4, 500rpm for 10min. in a refrigerated centrifuge at 0°C. The supernatant fluid was used as tissue extract. Lysozyme activities in these specimens were measured.The results obtained are as follows:1) The average activity in serum, mandibula, femur and femur marrow were respectively 8.4±0.5ug/ml, 42.1±2.4ug/g, 88.1±5.5ug/g and 626.0±34.6ug/g. The activity was higher in marrow than in any other tissue tested, though it showed a considerable variation.2) Lysozyme chloride was injected into the intra-peritoneal of the rat. After the intra-peritoneal injection of lysozyme chloride, the activity was greater in serum than in any other tissue and after 3 hours, lysozyme activity in serum was increased about 4-5 times. The mean rate of increase of the activities in mandibula, femur and femur marrow was not so high as that of serum, but that of mandibula was the highest in those three tissues.From these results it might be suggested that the high activity of the lysozyme in bone marrow make some physiological effects on the tissue.