Abstract
O-Phosphoethanolamine (PEA) was determined by cation exchange high-performance liquid chromatography in trichloroacetic acid extracts of spleens. An in-stream fluorometric detection system by using o-phthalaldehyde permitted the rapid analysis of samples containing 11.3-680.4 nmol/ml of PEA.The PEA concentration in mouse, rat, guinea pig, porcine and cattle spleens was determined. Spleens studied were found to vary widely in their PEA concentration showing species differences.
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