Abstract
A very rapid lysis of several amylase producing strains of Bac. subtilis was observed, when they were grown aerobically by shaking culture and then left in a fairly anaerobic condition without further aeration (Fig. 1). This phenomenon is first recognized at the last stage of the log phase when amylase begins to appear in the medium, and can be observed thereafter until the cessation of the amylase produc-tion (Fig. 2). Therefore, apparently both phenomena, amylase production and anaerobic lysis, take place in the same growth phase. The effect of the variation of the growth medium on this phenomenon was examined. The anaerobic lysis was also observed after the cells were harvested, washed and resuspend-ed in the new medium or buffer (Fig. 3). Therefore, this phenomenon is not due to the external substance, but to the factor within the cell. The cells derived from the single spore survived after the heat treatment (100- for 30 min. ), and still showed the same phenomenon. Accordingly, the lysis by carried bacteriophages was excluded and the anaerobic lysis seems to be the hereditary character of these organisms. Several strains of Bac. subtilis, Bac. megatherium and Bac. mesentericus were examined as to their character of anaerobic lysis (Fig 4, 5). This phenomenon has been pronounced only in the amylase producing strains of Bac. subtilis.
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