Abstract

The composition and biosynthesis of sterols in the blue-green alga, Anabaena cylindrica, were investigated. 1) From the blue-greeen alga cultivated aseptically in a defined medium, sterols were isolated by the digitonin-precipitation method. 2) The identification of sterols was performed by gas-liquid chromatography and mass spectrometry. 3) The sterols isolated from the blue-green alga were composed of brassicasterol (90%), cholesterol (8%), 22-dihydrobrassicasterol (2%), and 22-dehydrocholesterol (<1%). 4) The incubation of the blue-green alga with acetate-1-14C gave both radioactive squalene and sterols. The results suggest that the enzyme systems for biosynthesis of sterol exist in the blue-green alga under investigation.

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