ВОЗДЕЙСТВИЕ ВИРУЛЕНТНЫМИ БАКТЕРИОФАГАМИ НА БИОПЛЕНКИ KLEBSIELLA PNEUMONIAE

Abstract

One of the most important problems of our time is the growing level of resistance to antibiotics among human and animal pathogens. In this regard, more and more attention is paying to alternative therapeutic agents. In the present work, the effects of the bacteriophage vB_KpnP_FZ12 on Klebsiella pneumoniae biofilms have been investigated. Klebsiella pneumoniae 315 strain and the bacteriophage vB_KpnP_FZ12 were used. To study the formation of biofilms in liquid nutrient media by populations of Klebsiella pneumoniae 315, 17…18-hour cultures of microorganisms in the Sform (third generation) were used. A suspension of bacteria at a concentration of 1×105 CFU/ml in a volume of 5 ml was mixed on machine Vortex and introduced into Petri dishes with 20 ml of BHI broth. Sterile slides were placed on the bottom of a Petri dish, sterile fat-free cover glasses were placed on top, and incubated in a thermostat for 24-48 hours at 37°C. After 24 hours, 0.1 ml of the bacteriophage vB_KpnP_FZ12 was added with a concentration of 1×106 PFU/ml, incubated in a thermostat for 24 hours at a temperature of 37°C. In parallel, control glasses were incubated without adding phage. To preserve the natural architectonics, the samples were fixed in vivo in vapors of 25% glutaraldehyde for 3...5 h. It was shown that the bacteriophage is able to effectively destroy the structure of biofilms and clusters, while reducing the total number of bacteria.