Abstract
In isolated rat heart mitochondria incubated with excess ADP and malate, α-ketoisocaproate (KIC) at 0.05–0.1 mM more than doubled the rate of citrate cycle activity (measured as citrate accumulation in the presence of fluorocitrate). Replacement of fluorocitrate with arsenite increased pyruvate formation stoichiometrically with the previous increase in citrate formation. No enhancement of pyruvate or citrate accumulation was seen when malate was omitted or when KIC was replaced with equimolar leucine. KIC (leucine) did not give rise to acetyl CoA, nor was pyruvate formed by way of oxaloacetate and phosphoenolpyruvate. These findings suggest that KIC regulated the formation of pyruvate from malate by acting as a positive modulator of mitochondrial malic enzyme.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
