養殖アマノリの疾病に関する研究-IV

Abstract

Pathophysiological studies were made on canceraffected Porphyra and the carcinogenic effect of some chemicals was studied. The results are as follows: 1) The cancerous tissue showed altered nitrogen metabolism; the total nitrogen and the water-soluble protein content per cell decreased but NaCl- and Tris buffersoluble protein contents increased slightly. There was no difference in the electrophoretic pattern of the soluble proteins among both the healthy and canceraffected tissues. 2) The DNA content in the canceraffected tissue, expressed on a dry weight basis, was greater than that of normal tissues. However, when plotted on a fresh weight basis, no such difference was noted. 3) The concentrations of chlorophyll, phycoerythrin and phycocyanin were markedly decreased in the cancered tissue. 4) Respiration rate in the canceraffected tissue was greater than that of normal tissues. Respiration rate was however decreased when glucose was added as a substrate. This result indicates that the CRABTREE effect was induced by infection and fermentation occured in the cancered tissue. 5) It was possible to produce abnormally swollen cells or cancerous cells on Porphyra fronds by cultivating them in media containing KCN, As2O3 and phenyluretane respectively. 6) These results suggest the possibility that an irreversible damage to the respiratory system of the tissues could play an important role in the induction of cancer cells in Porphyra species.