Abstract

Four melon Inbred lines, belonging to var, reticulatus and maukwa and their progenies were used for studying isozyme variation in either seeds or young cotyledons. Among ten enzymes investigated, two were found to be polymorphic. They are peroxidase (PRX) and shikimate dehydrogenase (SkDH). For PRX, variety PMR has the phenotype of 2B2B, while Hsi-Shih, Hwang-Jin, and Shin-Yuh belong to 2A2A type. For SkDH, varieties PMR and Hsi-Shih have the phenotype of SS, while Hwang-Jin and Shin-Yuh belong to FF type. Samples from self ing progeny of a PMR×Shin-Yuh cross were analysed for segregation in SkDH locus. The result showed that it was controlled by a single locus of two alleles, each encoded a single band of different mobility. Sim-ilarly, PRX-2 may also found to be controlled by a single locus of two alleles, each expressed different three-banded pattern. The two genes were named as Skdh and Prx-2, respectively. Hybrid between genotype Prx-2A2A and 2B2B revealed four banded patterns, which indicated the codominance nature of the enzymes. Seed purity in hybrid could be monitored between two parents having different isozyme patterns.

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