Создание и поддержание коллекции хризантемы в культуре in vitro

Abstract

The collection contains more than 40 commercial varieties. It is shown that the optimal medium for in vitro storage and rooting of most varieties is the nutrient medium ¼ MS without the addition of growth regulators. The chrysanthemum microplants can be conserved on this culture media without subculturing up to 6 months under the temperature of +10 оС and light intensity of 20 lux. The period between passages is 2-3 months when plants are maintained under normal conditions (t + 20 оС and light of 3000 lux) using the same culture media. Addition of mannitol to the nutrient medium (100 and 200 mmol), inhibited shoot growth 2 and 5-times, respectively, compared with the control. The relative water content in the leaves was signifi cantly reduced under effect of mannitol in the nutrient medium. The proline content in the leaves was 3.42 and 4.65 mg / g (100 and 200 mmol of mannitol, respectively), which is 6-8 times higher than in the control. The obtained results allowed to improve the methods of cultivation of chrysanthemum in vitro.