Изучение процесса магнитоуправляемой доставки наночастиц магнетита в модельной системе in vitro

Abstract

Purpose. The development and validation of experimental setup for the investigation of the process of magnetic nanoparticle (MNP) accumulation in the specific area of the vessel model under the influence of external magnetic field. Materials and methods. Distilled water was used as a model fluid. The water was circulating in the closed loop at a rate of 40-80 L/h. The water was supplemented with magnetite nanoparticle suspension (diameter 7-10 nm) at concentration of 2 mg/ml. The morphology, specific surface area and magnetic properties of MNPs were evaluated. The time to accumulation of MNPs on the inner surface of the tube was recorded after application of constant magnetic field from the Nd magnet (N35) with the dimensions 30x20x10 mm. Results. The technology of MNPs synthesis, as well as their physicochemical properties were described. MNPs were characterized by the needle-like shape and surface area of 95 m 2 /g. It was shown that 100 % of MNPs from the circulating fluid are concentrated in the area of magnet application within 5 min after turning on the field. The MNPs were predominantly accumulated on the edges of the constant magnet, which is explained by the topography of the magnetic field. Conclusion. We developed the in vitro model setup for investigation of magnetic field-targeted nanoparticle delivery. Two types of MNPs introduction into the system could be used: direct MNPs injection in the circulation or introduction of the nanoparticles using special magnetic probe. The model setup suggested may be useful in studying the effectiveness of magnetically guided MNP transport depending on the volume flow of the fluid, salt composition, and viscosity.