二硫化炭素の代謝に関する研究 : III 二硫化炭素の代謝産物の証明方法の実験的研究

Abstract

The study on the metabolism of carbon disulfide (CS2) in the human body was already reported by the author. The present report is concerned with the metabolism of the retained CS2 in the rabbits. Nine rabbits were bred under the same conditions and they were divided into two groups of four and five animals; the CS2 exposed groups and the control. The experimental period lasted for ten days, during which middle four days the CS2 group was exposed to CS2 vapor of about three to four hundred p p m for eight hours a day. Total sulfate (SO3), nitrogen (N), and creatinine were analyzed in the urine which was collected for every twenty four hours and the nitrogen/total sulfate sulfur ratio (N/SO3-S) was calculated. The decrease of N/SO3-S ratio was understood to indicate the increase of SO3-S which was a metabolite of carbon disulfide retained in the animal body. The amounts of the total SO3-S, creatinine, and N/SO3-S ratio were statistically compared between the CS2 and control groups with following results. 1. The difference in the amounts of urinary excretion of total SO3-S and creatinine was not proved to exist between the CS2 exposed and the control groups. 2. N/SO3-S ration in the urine of the CS2 group were shown to decrease significantly by exposure to CS2 vapor. 3.The decrease of N/SO3-S ratio in the CS2 became apparent one day after the group exposure, and the decreased level was maintained for two days after the interruption of the exposure. 4. It was concluded that the retained CS2-S in the rabbit was metabolized to sulfate-S and that the rate of metabolism was relatively slow.