Abstract
It is the purpose of this series of the experiments to study on the rate limiting factors in glycolysis of the muscle of aquatic animals. In the present paper, the effects of substrates and cofactors on aerobic lactic acid formation or pyruvic acid formation in the crude muscle extracts are described. With the extract from the skeletal muscle of carp, it was demonstrated that fructose diphosphate (FDP) is the most requisite substrate for lactic acid production and the increasing amounts of added FDP stimulate proportionally the lactate formation, but in the presence of pyruvate, lactic dehydrogenase appeared to be limiting factor. DPN was more effective than ATP and the results of lactate formation when DPN was omitted are the same as in the absence of both. On the other hand, with the mantle muscle of squid, it has been observed that lactic dehydrogenase activity was very low and pyruvic acid was markedly accumulated as the end product of aerobic glycolysis. It may reflect different properties of enzymes of TCA cycle or an alternate route of pyruvic acid utilization. However, the effects of cofactors and substrates on the accumulation of pyruvate are the same as in the case of carp muscle.
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