Abstract

We evaluated the newly designed fiber-optic measurement system to measure human embryo viability. We measured angular distribution of the light scattering intensity to measure the embryonic mitodchondrial density in which localization may relate to the developmental ability. The size selectivity of the system was investigated with the latex sphere suspensions. The measured light scattering of these suspensions showed good agreement with the calculated results of which scattering distribution were differerent according to the latex diameter. We measured the light scattering of live mouse embryo. Since the measured scattering patterns of the embryo were similar to the pattern abtained from mitochondria equivalent particle, we think the measured light scattering manly originated from mitochondria in the live embryo. Our measurement method might become the practical selecting method of embryo to suppress the multiple pregnancy on current in vitro fertilization-embryo transfer.

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