Abstract
In the previous paper1), we reported that the sea bottom mud, heavily polluted by wastes discharged from the coal chemical industry in Ohmuta city in Kyushu, induced a certain cancerous disease to marine alga, Porphyra tenera after a single contact for only 80 to 320 min. and successive 36 day culture. In the present study, acids, neutrals and bases were separated from the same mud and subjected to carcinogenesis tests using very young Porphyra tenera. The tests revealed the carcinogenic effect of the mud to be mainly attributable to the neutrals in it. The neutrals were separated into nine fractions by repeated thin layer chromatography utilizing silica gel containing 5% gypsum for the stationary phase and n-hexane-ether 19:1 mixed solvent as the mobile phase, and two compounds were found to be carcinogenic to this alga; one, the yellow crystals with green fluorescence having an Rf value of 0.20 and another, an orange liquid with light blue fluorescence possessing an Rf value of 0.82.
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