Abstract
In the isolation of arginine from extractive matter of pyloric coeca, it is necessary to find means for elimination of agmatine. Basic nitrogen compounds containing arginine and agmatine were separated from other matters by the treatment with activated charcoal at pH 8.0 followed by the elution with HCl-acetone. After removed of acetone the residue was treated with picric acid, almost all the agmatine being precipitated. After filtyering off the agmatine picrate, arginine was precipitated as its flavianate, and finally purified as the hydrochloride. The yield of arginine thus obtained corresonded to 76% by the bioassay value.
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