Abstract
Phospholipids were extracted with chloroform-methanol from the egg of northern blenny, Stichaeus grigorjewi HERZENSTEIN. By adding a large amount of ethanol to these phospho-lipids, lecithin and cephalin fractions were separated. The lecithin fraction was first separated through a stage of cadmium chloride complex, and then purified by the alumina column chromatography. The results obtained in this experiment are shown in Tables 1-2 and Figure 1. As shown in Table 3, the fatty acid composition of lecithin consisted of palmitic, stearic palmitoleic, and oleic acid as high as 24.7, 13.8, 13.6 and 17.8%, respectively. Thus it may be concluded that the lecithin contained lower amounts of unsaturated acids than the acetone-soluble lipid.
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