Abstract
Choline-content was estimated for white and red muscles and viscera of various fishes of migrating and demersal habits, including sharks and cuttle fish. Its seasonal variation was also measured, values fairly characteristic of each fish being obtained. The amounts of choline and trimethylaminoxide(TMAO) increase generally in winter for demersal fishes and in summer for migrating ones. Decrease of freshness makes the chief cause for the increases of choline and trimethylamine (TMA) in common with different kinds of fishes. In cuttle fish and sharks, choline and TMAO contents were especially of large values, their rates of change being somewhat remarkable. Similarly to the case of salt-water fish, the choline content in fresh-water fish increased at first but decreased afterwards with the advance of putrifaction. It is noticed that TMA was formed even in the absence of TMAO, the known mother substance of TMA. The choline formation in this study was confirmed to be a bacterial doing.
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