Abstract

In order to investigate the photosynthetic activity of tobacco plants with special reference to the relationship between this activity and the content of cytochrome f, the method of extraction and some properties of this compound was studied.Cytochrome f was extracted from tobacco chloroplasts, using ammoniac ethanol solution containing Triton X-100. Tobacco cytochrome f had properties similar to those of parsley cytochrome f.The concentration of cytochrome f in the extract was 0.18 to 0.2μmoles and not affected by the variety of tobacco plants. As the concentration of chlorophyll of tobacco plants varied remarkably among the varieties, the ratio of chlorophyll/cytochrome f differed due to the difference of the starting materials.

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