Abstract
The contractile activity of urinary bladder (UB) after introduction of biologically active compositions (BAC) containing neurotrophic factors in female rats with infravesical obstruction, which was modeled by a ligature to the urinary tract distal part, was investigated. Cryoextract of the spinal ganglia (SG), conditioned culture media of native and cryopreserved mantle gliocytes (MG), the bulk medium, the drug Cortexin were used as BAC. Animals were injected with BAC for 10 days and the contractile activity (CA) of isolated UB strips was determined by measuring KCl-induced and agonist-dependent responses. The use of BAC, in addition to Cortexin, contributed to the CA restoration. The conditioned culture medium of cryopreserved MG and cryoextract of SG increased the agonist-dependent response by 4.6 and 2.5 times, respectively, compared to the CA of the control animals. The rats in these groups showed the highest indices of maximal amplitude of contraction, similar to the control ones. Probl Cryobiol Cryomed 2020; 30(2): 188-198
Highlights
The contractile activity of urinary bladder (UB) after introduction of biologically active compositions (BAC) containing neurotrophic factors in female rats with infravesical obstruction, which was modeled by a ligature to the urinary tract distal part, was investigated
Indices of KCl-induced and pilocarpine-dependent reduction of UB isolated strips (IS) of intact rats and rats with Infravesical obstruction (IVO) after administration of different types of BAC and Cortexin (Me (Q1; Q3); n = 57)
In this research we studied an initial, 1.5-month obstruction period characterized by an initial increase in UB mass and lower detrusor contractility (DC) in response to agonist stimulation compared with control animals [21]
Summary
The contractile activity of urinary bladder (UB) after introduction of biologically active compositions (BAC) containing neurotrophic factors in female rats with infravesical obstruction, which was modeled by a ligature to the urinary tract distal part, was investigated. The conditioned culture medium of cryopreserved MG and cryoextract of SG increased the agonist-dependent response by 4.6 and 2.5 times, respectively, compared to the CA of the control animals. The rats in these groups showed the highest indices of maximal amplitude of contraction, similar to the control ones. Infravesical obstruction (IVO) can cause the UB dysfunction as a result of a decrease in detrusor contractility (DC), neuronal degeneration and partial denervation in smooth muscles, disruption of the neural network in the central nervous system (CNS) [11, 13, , 23, 40]
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