エノキタケの遣伝学, I. 交配型とバラージュ現象 (2)

Abstract

A mating experiment using the twenty-one monosporous mycelia of Collybia velutipes shows that this fungus is heterothallic and tetrapolar (Table 1). The foregoing result confirms the statements of Vandendries (1923), Zattler (1924), Heldmaier (1930), and others regarding the mating type of the fungus. Twelve months after these monosporous mycelia had been isolated, the spontaneous dikaryotization were examined. Twenty of the twenty-one monosporous mycelia remained in the monokaryotic condition. It is concluded that the fungus is stable for mating type, and that the “hétéro-homothallisme” theory advanced by Vandendries (1925) is hardly applicable to the fungus. In some of the combinations between the single-spore mycelia of C. velutipes, the barrage phenomenon as shown in Figs. 5-9 was observed. The barrage manifested between the monosporous mycelia of the fungus, however, exhibited a very irregular behaviour as shown in Table 2. The theory advanced by Vandendries and his collaborator in Lenzites betulina and other hymenomycetous fungi that the barrage appears only between any two mycelia which are heterozygous in one of the two pairs of mating type alleles independently of the other pair of the alleles cannot be applied to C. velutipes. It is shown to be possible to control the conditions of culture in such a way that the fruit-bodies of the fungus can be developed aseptically in a glass container as desired. Most parts of the present experiments were achieved from April 1950 to March 1951 for the graduation thesis of the University of Tokyo, and it is a great pleasure for the writer to acknowledge his indebtedness to Professor Y. Sinotô and to Dr. N. Tanaka for their cordial leadership during the work. Thanks are due also to Professor H. Ono for his encouragement and very helpful suggestions. He also wishes to thank Professor K. Dan for his correction of manuscript. Further he is indebted to Mr. B. Sakai for making photographs. Finally he wishes to express gratitude to Mr. K. Aoshima, who kindly provided him with the original culture.