マウスから分離された赤血球凝集性のウイルス (HVJ, Z株について第II報)

Abstract

Several characteristics of HVJ., Z strain, not described in the previous report were presenting here.1. Hemolytic action was revealed when Z virus was eluted from fowl red blood cells. The intensity of the hemolysis increased with the furtherance of the virus concentration.The hemolytic activity could be blocked with the homologous immune sera and treatment of the virus with formaline showed a reduction of the hemolytis. The inactivated virus obtained as a result of the destruction of the enzyme activity by heating revealed no hemolysis.The intensity of the heinolysis was compared with the infected allantoic fluid and purified virus suspension in buffered saline. The hemolysis of the purified virus was stronger than the infected allantoic fluids which had the same titer with the hemagglutinating activity. The hemolytic activity was hightened by adding a small amount of ethylene diamine tetraacetate disodium or sodium citrate.To get the maximum hemolysis in the infected aflantoic fluids 40 to 50 times as much EDTA as the purified virus suspensions was required.The hemolysis was blocked by adding calcium chloride. The normal allantoic fluids inhibited the herolysis also.2. Multiplication of the Z virus in chick embryos was held in check when it was injected with the active or irradiated influenza viruses, either simultaneously or at different times.a) About 1 EID50 of the active Z virus was excluded by the succeeding inoculation of the irradiated Lee virus of 100 EID50 equivalent.b) The active Lee virus and Z virus were mixed in ratio 1:1 and the mixtures was injected into chick embryos in dilution of 10-1 and 10-3. In the first mixture, the Lee exceeded the Z. In the second mixture, the reverse wsa true.c) The Z and PR8 strain were mixed in varying proportions. The data showed that the Z virus and PR8 virus carry on interference between the two viruses, and under certain conditions both the X virus and PR8 strain can be multiplied simultaneously.