Abstract
The method of extraction from vegetable raw materials, allowing to receive non-toxic composition of biologically active substances from poisonous plants containing flavonoids, for example, poisonous plant coast of Gratiola officinalis L. The increase of the percentage content of ethyl alcohol (from 15 to 96%), used as a solvent, output changes alkaloids so that the extract obtained 96% ethanol does not provide for positive qualitative reaction on the content of alkaloids. Investigated the chemical composition of the extract of the coast of Gratiola officinalis non-toxic, biologically active composition. It is established that the extract contains a previously unknown to this plant bioflavonoid - quercetin. The average value of quercetin in this extract, using the calibration curve of the standard sample quercetin (98%) Sigma, is 0.66%. Established by liquid chromatography (HPLC) number of quercetin in the dry residue of the extractive substances (gained from 10 g of dry grass coast of Gratiola officinalis) amounted to 350 mcg.
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