Abstract
GmPGIP3 is a polygalacturonase-inhibiting protein from soybean, which could reduce the infection of fungal pathogen throughr inhibiting the endo-polygalacturonase activity of pathogen fungi. Using genetic recombination technique, we constructed the transformation vector of GmPGIP3 gene expressing highly in monocot plants, pA25-GmPGIP3, in which GmPGIP3 gene was driven by maize ubiquitin promoter. Embryo callus of Yangmai 18 was bombarded by the particle containing pA25-GmPGIP3 vector DNA. The GmPGIP3 transgenic wheat plants from T 0 to T 2 generations were detected by PCR, Southern blot, RT-PCR, and Q-RT-PCR analyses. The alien GmPGIP3 proved to be introduced into seven transgenic wheat lines with heritability and expression events. We also evaluated the disease resistance in these GmPGIP3 transgenic plants through inoculating the common root rot pathogen, Bipolaris sorokiniana . Compared with untransformated Yangmai 18, five GmPGIP3 transgenic lines showed significantly-enhanced resistance to Bipolaris sorokiniana.
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