Abstract
The carbohydrate and amino acid composition of non-dialyzable β-glucuronidase isolated from guinea pig urine was determined. The carbohydrate composition was hexose 9.5%, methylpentose 3.6%, hexosamine 14.2%, uronic acid 7.1%, and sialic acid 2.3%. The mo1ar ratio of mannose to galactose was 1 : 1 and the hexosamine consisted of both glucosamine and galactosamine in a ratio of 5 : 1. The polypeptide portion of the inhibitor consisted of 70 amino acid residues according to the amino acid analysis. No aromatic or S-containing amino acids were present in the inhibitor, and a small amount of sulfate group was detected in it. On the basis of these results, it was concluded that the non-dialyzable β-glucuronidase inhibitor was a glycopeptide composed of a carbohydrate, amino acids, and a sulfate. Molecular weight of about 10000 was estimated for this inhibitor from the amino acid analysis and gel filtration.
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