β-Glucuronidase の生化学的並びに組織化学的研究

Abstract

Recently, relationship between the β-Glucuronidase activity and the various tumors has been in vestigated by many workers.Biochemical determination of β-Glucuronidase activity in serum, urine and pathological tissue homogenate of patients suffering from various urological diseases was performed by the Talalay, Fishman and Huggins' procedure. And of the pathological tissue, histochemical staining of this enzyme was carried out by the Burton and Pearse's method.The results were as follows:1. In the patients with malignant tumor, for instance, bladder carcinoma, prostatic carcinoma and testicular tumor, β-Glucuronidase activity was higher than in the normal subjects. From these facts, the determination of this enzyme activity in serum and urine has proved to be useful for diagnosis of the malignant tumor in urological field.2. It was found in the histochemical studies that intensive activity was revealed in the proximal convoluted tubules and the Henle's ascending limbs of the normal kidney, in epitherial layers of the prostatic gland, in papillary tissue of the bladder tumor, and also in the area where the infiltration of malignant tumor cell was marked.3. From these results, the rôle of this enzyme is thought to be a catalyzer for the processes from the mucopolysaccharide to the nucleic substances, especially in the tumor tissue.