Abstract
Tofu industrial waste has the potential to become contaminant when it is not maximum processed, so it needs appropriate treatment to minimize the occurrence of pollution. β-Glucans can be produced from the yeast cell wall of Saccharomyces cerevisiae.As a feed supplement, β-Glucans have been used for an immunostimulant because it can increase the immune system. This study aimed to establish a suitable technology to produce β-Glucans fromSaccharomyces cerevisiae by utilize tofu waste as feed supplement. The Completely Randomized Design (CRD) model was used in the experimental method. This study used an experimental method with a Completely Randomized Design (CRD) model.The treatment given wereP1: 1kg of tofu waste + 1kg of bran +0.2 kg fish meal + 0.2 lt molasses + 5.5 gSaccharomyces cerevisiae; P2: 1kg of tofu waste + 1kg of bran +0.2 kg fish meal + 0.2 lt molasses + 6.5 g Saccharomyces cerevisiae; P3: 1kg of tofu waste + 1kg bran + 0.2 kg fish meal +0.2 lt molasses + 7.5 g Saccharomyces cerevisiae. Each treatment was repeated 3 times, and fermented for five days.The results found that P1 by Saccharomyces cerevisiae 5.5 g was the best formulation to produced crude β-Glucans by weight of 25.9 g/kg of waste. Production of β-Glucans as an animal feed supplement is a waste-based feed technology development which is expected to increase livestock production both in quality and quantity.
Highlights
The tofu industry is one industry with large waste production
Tofu waste is used as a substrate for the growth of Saccharomyces cerevisiae, while sugar cane waste is used as a source of glucose to increase production of Saccharomyces cerevisiae cells (Mufarida & Probowulan., 2019)
The use of waste is intended as an effort to minimize the occurrence of environmental pollution.The solid waste of the tofu processing plant is in the form of soybean cleaning and the rest of the soybean slurry called tofu pulp
Summary
The tofu industry is one industry with large waste production. The waste produced is liquid waste or solid waste, which if dumped directly into the environment will result in a very large impact of environmental pollution. The process will use dissolved oxygen in water, so that the availability of oxygen for life in the environment decreases. This can bring the death of living things in. Saccharomyces cerevisiae has cell walls containing β-D-Glucans, chitin, and manoprotein. The findings targeted in this study are new appropriate technologies, namely waste-based "feed supplement" products containing β-Glucansthat can be accepted and applied by farmers to increase fish production. Existing fermentation technology innovations have not maximally been able to produce feed supplements that are rich in β-Glucans as fish immunostimulants. The purpose of this study was to determine the right concentration of Saccharomyces cerevisiae which can contribute optimally to the production of β-Glucans in wastebased feed supplements
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