Abstract

2006년부터 2007년까지 벼키다리병균을 수집하여 25개 균주의 prochloraz와 benomyl + thiram에 대한 약제저항성을 검정하였다. 공시한 균주 중, 대부분의 균주가 감수성이었으나 prochloraz에 대한 약제저항성 균주는 6개로 조사되었고, benomyl + thiram에 대해서는 3개 균주가 저항성을 나타내었다. 이 중 CF245, CF249, CF337은 공시약제 모두에 대해 저항성이었다. 감수성 균주간 교배 및 감수성, 저항성 균주간 교배를 통해 얻어진 후대균주를 분리하여 온도별 균사 생장율과 약제에 대한 반응을 조사하였다. 온도별 균사 생장은 감수성 균주간 교배(CF202 <TEX>$\times$</TEX> CF232)에서는 모균주와 비슷하였으나, 감수성과 저항성 균주간 교배(CF179 <TEX>$\times$</TEX> CF337)에서는 모균주에 비해 후대균주의 균사생장이 빠른 것으로 나타났다. 공시약제에 대한 반응은 약제에 따라 모균주와 다른 양상을 나타내었다. Prochloraz의 경우, 감수성균주인 CF202 <TEX>$\times$</TEX> CF232의 후대균주에서 감수성과 저항성의 분포비율이 86 : 14로 나타났고, 감수성과 저항성 균주인 CF179 <TEX>$\times$</TEX> CF337의 후대균주에서는 78 : 22로 나타났다. Benomyl + thiram의 경우, CF202 <TEX>$\times$</TEX> CF232의 후대균주는 모두 감수성인 것으로 나타났으며, CF179 <TEX>$\times$</TEX> CF337의 후대균주에서는 35 : 65로 조사되었다. A total of 25 isolates of Fusarium fujikuroi were obtained from diseased rice plants in Korea from 2006 to 2007 to assess their resistance against fungicides prochloraz and benomyl + thiram. Minimal inhibitory concentration (MIC) values of F. fujikuroi isolates were examined by agar dilution method. Most of the isolates were sensitive to the fungicides. Out of 25 isolates, six were resistant to prochloraz and three to benomyl + thiram. In addition, the isolates CF245, CF249 and CF337 showed resistant to both fungicides. The progenies (<TEX>$F_1$</TEX> isolates) obtained through two different crosses between sensitive parental isolates(CF202, CF232 and CF179) and resistant parental isolate (CF337) were evaluated for their mycelial growth at different temperatures and resistance against fungicides. Mycelial growth rate of <TEX>$F_1$</TEX> isolates originated from CF202 <TEX>$\times$</TEX> CF232 was similar to the parental isolates. However mycelial growth rate of <TEX>$F_1$</TEX> isolates originated from CF179 <TEX>$\times$</TEX> CF337 was faster than their parent isolates. In case of prochloraz, distribution ratio of sensitivity(S) to resistance(R) against to the fungicide of <TEX>$F_1$</TEX> isolates originated from CF202 <TEX>$\times$</TEX> CF232 and CF179 <TEX>$\times$</TEX> CF337 was 86 : 14 and 78 : 22, respectively. In case of benomyl+thiram, all the <TEX>$F_1$</TEX> isolates originated from CF202 <TEX>$\times$</TEX> CF232 were sensitive to the fungicide, however ratio of sensitivity(S) to resistance(R) against to the fungicide of <TEX>$F_1$</TEX> isolates originated from CF179 <TEX>$\times$</TEX> CF337 was 35 : 65.

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