ВПЛИВ ЛАЗЕРНОГО ОПРОМІНЕННЯ НА КАТАЛАЗНУ АКТИВНіСТЬ БАЗИДІОМІЦЕТА FLAMMULINA VELUTIPES (CURT.: FR.) SING.

Abstract

It has been studied the effect of laser irradiation of different spectrum on the catalase activity of the culture filtrate and the mycelium homogenate of 5 strains of basidiomycetes Flammulina velutipes (Curt.:fr.) Sing. at their surface cultivation on a standard glucose–peptone medium. The irradiation of the inoculum sized approximately 5x5 mm (always the same density and age) was carried out before the inoculation with the help of such LED lasers: BRP–3010–5 with red laser light (wavelength 635 nm), BBP–3010–5 with blue laser light (wavelength 405 nm) and BGP–3010–5 with green laser light (wavelength 532 nm). Each laser power was 100 MW. The irradiation energy at all the experiment variants was 51.1 MJ/m 2 . The 10–day mycelial culture of the strains on wort–agar served as inoculum. The non–irradiated mycelium was used for the control inoculation. The catalase activity in the mycelium homogenate and the culture filtrate was defined spectrophotometrically. It was found out that the laser light of red, blue and green spectra for 10 s caused the increase of the catalase activity of the culture filtrate and the mycelium homogenate of all the strains under research. The effect of complex laser irradiation of red and green spectra does not cause probable changes of the ferment activity of the strains under research. The biggest reaction to blue laser light had the strains F–04 and F–vv of F . velutipes . Thus, the indicator of the catalase activity of the culture filtrate for the strain F–04 was 780.41±15.46 mcat/l, the maximum value of the catalase activity of mycelium for the strain F–vv of F. velutipes was 1203.66±20.31 mcat/g. The other strains under analysis showed less significant catalase activity change in response to irradiation. The research carried out allowed to determine the general consistent patterns and individual peculiarities of different strains of F . velutipes reactions to irradiation spectrum and to identify the sufficient laser photoactivation mode greatly increasing the catalase activity of studied macromycetes.