Abstract

본 연구는 마늘의 물추출물(AGE)의 E. coli O157:H7, S. typhimurium, 그리고 S. aureus에 대한 항균효과를 조사하였다. AGE의 E. coli O157:H7, S. typhimurium, and S. aureus에 대한 최소억제농도(MIC)는 각각 24, 48 그리고 24 mg/mL이었으며, 최소살균농도(MBC)는 모든 균에 대하여 96 mg/mL이었다. AGE 24 mg/mL을 E. coli O157:H7에 투여하고 배양 24시간 후에, 균의 증식이 무처리 대조군과 비교하여 유의성 있게 감소하였다(p < 0.01). 그러나 AGE 24 mg/mL을 S. aureus에 투여하고 배양 24시간 후에, 대조군과 비교하여 유의성 있는 균의 증식억제 효과가 나타나지 않았으나, AGE 96 mg/mL을 투여한 경우에서는 배양 24시간 후에 대조군과 비교하여 유의성 있는 균의 증식억제가 나타났다(p < 0.01). AGE 48 (p < 0.05)과 96 mg/mL (p < 0.001)을 S. typhimurim에 투여하고 24시간 배양 후에, 대조군과 비교하여 통계적으로 유의성 있는 균의 증식억제 효과가 나타났다. 본 연구의 결과로부터, 마늘의 물추출물은 항생제와 화학적 식품보존제의 대체제로서 사용이 가능할 것으로 사료된다. This study was investigated about the antibacterial effects of aqueous garlic extract (AGE) against Escherichia coli O157:H7 (E. coli O157:H7), Salmonella typhimurium (S. typhimurium) and Staphylococcus aureus (S. aureus). The minimum inhibitory concentration (MIC) of AGE against E. coli O157:H7, S. typhimurium, and S. aureus was 24, 48 and 24 mg/mL, respectively. In addition, the minimum bactericidal concentration (MBC) of AGE against E. coli O157:H7, S. typhimurium, and S. aureus was all of 96 mg/mL. The growth of E. coli O157:H7 was significantly inhibited at the concentration of AGE 24 mg/mL at 24 hr post-incubation (p < 0.01), but that of S. aureus was not significantly inhibited at the same concentration. However, the growth of S. aureus at the concentration of AGE 96 mg/mL was significantly inhibited at 24 hr post-incubation compared to that of untreated bacteria (p < 0.01). At the concentration of AGE 48 (p < 0.05) and 96 mg/mL (p < 0.001), the growth of S. typhimurim was significantly inhibited at 24 hr after incubation compared to that of untreated bacteria. With the results of this study, AGE can be used as alternative to antibiotics and chemical food preservatives.

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