연교김패전(連翹金貝煎)의 Escherichia coli에 대한시험관내 항균력 평가

Abstract

ABSTRACT In Vitro Antibacterial Effects of Yeonkyokeumpae-jeon against Escherichia coliSang-Kyum Han, Dong-Chul KimDept. of Korean Obstetric & Gynecology, College of Korean Medicine,Deagu Haany UniversityObjectives: The object of this study was to observe the in vitro antibacte rial effects of Yeonkyokeumpae-jeon (YKKPJ) have been used for treating various gynecological diseases including mastitis in K orea, and individual six kinds of herbal composition aqueous extracts - Forsythiae Fructus (FF), Millettiae Caulis (MC), Lonicerae Flos (LF), Fritillaria Thunbergii Bulb (FT), Taraxci Herba (TH) and Prunellae Spica (PS) against E. coli. Methods: Antibacterial activities against E. coli of YKKPJ, FF, MC, LF, FT, TH and PS aqueous extracts were detected using standard agar mi crodilution methods. In addition, the effects on the bacterial growth curve were als o monitored at MIC and MIC×2 levels. The effects on the intracellular killing and bacterial invasion of individual test materials were also observed using Raw 264.7 and MCF-7. The results were compared with ciprofloxacin, a second generation o f quinolone antibiotics in the present study.Results: MIC of YKKPJ, FF, MC, LF, FT, TH, PS aqueous extracts against E. coli were detected as 0.039 ±0.013 mg/ml, 0.064±0.033 mg/ml, 0.108±0.053 mg/ml, 0.078±0.027 mg/ml, 16.250±8.385 mg/ml, 15.625±9.375 mg/ml, 0.254±0.131 mg/ml, repectively. YKKPJ, FF, MC, LF, FT, TH, PS aqueous extracts sho wed antibacterial effects against to E. coli, except for FT and TH, which were showed negligible antibacterial effects, respectively. In addition, ciprofloxacin with YKKPJ, FF, MC, LF and PS aqueous extracts also showed marked dosage-dependent inhibition of bacterial growth, and favorable inhibitory effects on the both bacterial invasion and intracellular killing assays using MCF-7 and Raw 264.7 cells were detected in this experiment. Conclusions: The results obtained in this study suggest that traditional po lyherbal formula YKKPJ aqueous extracts showed more favorable antibacterial activities as compared to individual six kinds of herbal composition aqueo us extracts. The antibacterial effects of YKKPJ against E. coli considered as results of complicated synergic effects of their six kinds of herbal components rather than simple antibacterial effects of single herbal components. It means, YKKPJ aqueous ex tracts may show potent anti-infectious effects against E. coil for mastitis.Key Words: Yeonkyokeumpae-jeon, Escherichia coli , Mastitis, Ciprofloxacin, Raw 264.7, MCF-7