Abstract
Male albino rats of the Donryu strain were divided into 4 groups : the normal control group, tetrachloromethane (CCl4, TCM) group, tetrachloroethylene (CCl2=CCl2, PCE) group and trichloroethylene (CHCl=CCl2, TCE) group. Each group was consisted of 6 animals.TCM, PCE and TCE were administered orally at a dosage of 13 mmoles/kg as a 50% v/v olive oil solution to rats 24 hours prior to an oral administration of m-xylene (8.2 mmoles/kg as a 50% v/v olive oil solution). The normal control group was administered only olive oil (2.5 ml/kg) 24 hours prior to m-xylene dose.Urine was collected 24, 48, 72 and 96 hours after administration of m-xylene. The urine was analysed for m-methylhippuric acid (m-MHA) by the paper chromatographic method of Ogata et al. The results obtained were as follows : 1) Total urinary excretion of m-MHA in the normal control group was equivalent on a molar basis to about 58% of m-xylene received, and the major portions of the metabolites were excreted on the 1st day.2) In TCM group, total urinary excretion of m-MHA was about a half of that in the normal control group. It was equivalent on a molar basis to about 30% of m-xylene received (p<0.01), and the urinary excretion of m-MHA on the 1st day was significantly less than those of the normal control group (p<0.001) and TCE group (p<0.05).3) In PCE group, urinary excretion of m-MHA on the 1st day was significantly less than that of the normal control group (p<0.01) and the excretion was delayed.4) In TCE group, total urinary excretion of m-MHA was slightly but not significantly less than that of the normal control group (p>0.05).5) In TCM-ip group of rats which were given TCM orally 24 hours prior to the administration of m-xylene by intraperitoneal injection, urinary excretion of m-MHA on the 1st day and in 4 days after administration of m-xylene was less than that of the control-ip group.
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