Abstract
The rotifers Brachionus plicatilis cultured with baker's yeast Saccharomyces cerevisiae (Y-rotifers), a living feed for fish larvae, are much lower in dietary value than those cultured with marine chlorella (C-rotifers). A trial was conducted in order to improve the low dietary value of Y-rotifers by feeding them marine chlorella Chlorella minutissima for 10min to 24hrs at regular intervals before they were given to red sea bream larvae. The dietary value of the secondary cultured rotifers in each period was compared with that of C-rotifers. The dietary value of Y-rotifers was found to be improved effectively by the secondary culture with chlorella. The larvae fed on Y-rotifers cultured secondarily for more than 6hrs showed good growth conditions and a high survival rate, comparable to those obtained by the larvae fed on C-rotifers. On the other hand, the dietary value of Y-rotifers was not much improved by the secondary culture with freshwater chlorella Chlorella reguralis.
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