ГЕНОМНЫЕ ИЗМЕНЕНИЯ У ПОТОМКОВ САМЦОВ DROSOPHILA MELANOGASTER, ОБЛУЧЕННЫХ y-КВАНТАМИ СО60

Abstract

The results of sequencing and bioinformatics analysis of genomic changes in 9 F1 progeny of males from the isogenic line D. melanogaster irradiated by Co60 Y—rays at a dose of 40 Gy (LD85) and in 3 control samples are presented. In 9 progeny from irradiated males, a total of 46 genomic changes (32 significant and 15 mosaic de novo mutations) were found, which is equal to a frequency of 5.2 mutations/genome. The spectrum of changes included 33 deletions (17-78 000 bp in size), 4 duplications (322-1371 bp), 4 reciprocal translocations and 6 inversions in X, 2 and 3 chromosomes. In 3 studied control samples, 2 deletions (98 and 128 bp in length) were found in 3 chromosome (frequency - 0.66 mutations/genome). This shows that in the progeny of irradiated males, the frequency of de novo mutations at the genome level is 7.9 times higher than in the control, even without taking into account base substitutions and indels, the analysis of which is ongoing. Almost half of the identified structural changes in the genome affect coding genes. Thus, the results show that next-generation genome sequencing can detect a much wider range of mutations of any size. This indicates a much higher genetic hazard of sparsely ionizing radiation than previously thought.