Abstract
NCBI GenBank nucleotide database and BOLD system provide DNA barcode sequences and tools to identify the taxonomic origins based on the sequence similarity and/or identity of BLAST(Basic Local Alignment Search Tool) analysis. However, these database did not verified its reality of the taxonomic origins for DNA barcode sequences by the confirmation of voucher specimens. To verify the application of DNA barcode sequence for molecular authentication of herbal medicines, we analyzed the sequence length, identity, concensus sequence, alignment range, and species specific nucleotide by the comparison of intra- and inter-species similarity using GenBank rDNA-ITS, matk, rbcL, and psbA-trnH sequences of Scuellaria species. This study would be a helpful reference to plan a scheme for the molecular authentication of herbal medicine based on the comparison of gene bank DNA barcode sequences.
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