Abstract
A study was made on the nuclear conjugation in diploidisation by the doubly compatible diploid mycelium, using Coprinus macrorhizus f. microsporus (a tetrapolar fungus). A small diploid mycelium was inoculated at the periphery of a large haploid mycelium. One of the nuclei of the diplont derived from the same fruit-body with the haplont and the other from a different fruit-body. Both nuclei of the diplont were compatible with the nucleus of the haplont. Nuclear constitution of the diploid mycelium resulting from the doubly legitimate combination was analyzed. The result showed that, in many combinations, the nucleus of the large haploid mycelium conjugated with that from the different fruit- body, but, in some combinations, with the nucleus from the same fruit-body. The above result may be well explained on the assumption of modifiers which control the affinity of conjugation between two compatible nuclei.
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