ビタミン D 欠乏が脛骨に及ぼす影響に関する X 線的, 光顕的および走査電顕的研究 : 骨基質形成の変化

Abstract

Rats were grouped into the standard food group, calcium deficient diet group, vitamin D deficient diet group, and calcium and vitamin D deficient diet group, and changes in bone matrix in the tibia was investigated, with the tibia of the rats in growing stage as specimen. The results were as follows : 1. Findings in bone density No significant difference (p>0.05) was found between the control group and the vitamin D deficient diet group. Among the control group, calcium deficient diet group, and calcium and vitamin D deficient diet group, significant difference (p<0.01) was found and the control group showed higher values. 2. Analytical findings on the X-ray microanalyzer In the quantitative analyses of Ca and P, relative Ca ratio and relative P ratio showed decreasing values in the order of the control group, vitamin D deficient diet group, calcium and vitamin D deficient diet group, and calcium deficient diet group. 3. Histopathological findings In contact microradiogram findings and general tissue findings as compared with those in the control group, the calcium deficient diet group and calcium and vitamin D deficient diet group showed increase in bone marrow in the tibia, decrease in Haversian canals and bone lacunae, and low calcified layers in nearly all bone layers. The tibia in the vitamin D deficient diet group showed about the same findings as those of the control group. 4. Scanning electron microscopic findings As compared with the control group, the calcium deficient diet group and calcium and vitamin D deficient diet group showed about the same findings, and bone resorption exceeded bone formation (area). Bone resorption was shallow in process at most sites. The calcium and vitamin D deficient diet group showed abundant findings of sparse collagen fibril network close to the resorption sites especially. The tibia in the vitamin D deficient diet group showed about the same findings as those of the control group. On the bases of the foregoing findings, calcium deficiency greatly affected the changes in the formation of bone matrix in the vitamin D deficient diet group.