Abstract
α-D-Glucopyranosyl fluoride (glucosyl fluoride) was found to be a substrate for sucrose phosphorylase from Pseudomonas saccharophila . At saturating P i (0.030 M) the apparent K m is the same as that of sucrose, but the apparent V max is 2.7 times as great; therefore, glucosyl fluoride is the best glucose donor presently known for sucrose phosphorylase. The enzyme catalyzes hydrolysis of glucosyl fluoride at the same rate as sucrose and glucose-1-P.
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More From: Biochemical and Biophysical Research Communications
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