Abstract
We have developed technology to identify and separate biological cells while they are alive and have fabricated the automated biological cell analyzing and sorting system “CS-5000”.The CS-5000 is a new flow-system instrument for quantitative analysis and sorting of microscopic particles, particularly biological cells, based on multiple measurements of physical and biological properties.Cells stained with fluorescent dyes in liquid suspension are jetted out through a micro-nozzle at a high speed and are illuminated by laser beam as they flow in a stream. Optical sensors measure light-scatter, single- or two-color fluorescence. Sensor signals are electronically processed in several ways for optimum cell discrimination and displayed as pulse-amplitude distribution using a pulse-hight analyzer. Sorting is accomplished by electrically charging droplets containing the cells and electrostatically deflecting them. into collecting vessels.Use of this system in biological and biomedical reseach and clinical medicine will greatly contribute to the further development of life sciences.
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