Abstract
Crocus sativus (saffron) contains biologically active substances and is used in the food and cosmetic industries. With the preservation of plant diversity in nature, the biotechnological method of tissue culture was used and the plant was introduced into in vitro conditions. The influence of the composition of the nutrient mediumon the growth of biomass and production was studied. The best results were obtained with the use of growth regulators benzylaminopurine (BAP) and naphthylacetic acid (NOK) in the amount of 20 μmol/l BAP + 15 μmol/l NOK. Cultivation time was 12 weeks. After transplanting into pots with clay soil, bulbs weighing 2.5 g sprouted and bloomed. The use of the biotechnological method of tissue culture is economically, ecologically beneficial and promising.
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