Abstract
The aim of this study is to investigate the potential inhibitory effect of α-chaconine and α-solanine on RL95-2 estrogen receptor (ER) positive human endometrial cancer cell line and to identify the effect of these glycoalkaloids on the Akt signaling and ERα. The cell proliferation profiles and the cytotoxicity studies were performed by Real-Time Cell Analyzer (xCELLigence) and compared with Sulphorhodamine B (SRB) assay. The effects of α-chaconine (2.5, 5, 10 µM), α-solanine (20, 30, 50 µM), API-1 (25 µM) and MPP (20 µM) effects on Akt (Ser473) and ERα (Ser167) expressions evaluated by Western blot and qPCR method. Their IC50 values were as α-chaconine (4.72 µM) < MPP (20.01 µM) < α-solanine (26.27 µM) < API-1 (56.67 µM). 10 μM α-chaconine and 20, 30 and 50 μM α-solanine were effective in decreasing p-Akt(Ser473)/Akt ratio compared to positive control API-1. When the p-ERα/ERα ratios were evaluated, it was observed that α-chaconine (2.5, 5, 10 μM) and α-solanine (50 μM) were as effective as the specific ERα inhibitor MPP in reducing the ratio of p-ERα/ERα compared to the control group. In conclusion, it has been shown that the proliferation of α-chaconine and α-solanine in human endometrial carcinoma cells reduces the expression and activity of the Akt and ERα signaling pathway.
Highlights
Glycoalkaloids, a class of nitrogen-containing steroidal glycosides, are biologically active secondary plant metabolites, usually found in plants of the genus Solanum
The aim of this study is to investigate the potential inhibitory effect of α-chaconine and α-solanine to clarify the potential of inhibiting proliferation, and to explain the effect of these glycoalkaloids on the Akt signaling and estrogen receptor α (ERα) in RL95-2, which is estrogen receptor (ER) positive human endometrial cancer cell line
When we examined the growth profile of RL95-2 cells, it was seen that the Cell Index (CI) values were low
Summary
Glycoalkaloids, a class of nitrogen-containing steroidal glycosides, are biologically active secondary plant metabolites, usually found in plants of the genus Solanum. These plants include many agricultural plants, especially the plants from Solanaceae family such as Solanum tuberosum L. S. tuberosum glycoalkaloids are naturally produced and the main glycoalkaloids are α-chaconine and α-solanine, which make up 95% of the total glycoalkaloid content. The molecules of both substances contain the same steroid scaffold (aglycone), but differ in the trisaccharide moiety [1].
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