황련해독탕(黃連解毒湯)이 외열 스트레스(화사(火邪))에 의한 동물모델에서 c-fos 발현에 미치는 영향 연구

Abstract

ABSTRACT The Effect of Hwangryunhaedok-tang on the c-fos Level in Mice Exposed to Heat StressDeok-Sang Hwang 1 , Hyo-Geun Kim 2,3 , Tae-Mi Kim 2, Tae-Hee Lee 4 , Myung-Sook Oh 2,31 Dept. of Korean Gynecology, College of Korean Medicine, Kyung-Hee University 2 Dept. of Life and Nanopharmaceutical Science, Graduates school, Kyung-Hee University 3 Dept. of Oriental Pharmaceutical Science, College of Pharmacy and Kyung-Hee East-West Pharmaceutical Research Institute, Kyung-Hee University 4 Dept. of Formulae Pharmacology, School of Oriental Medicine, Ga-Chon UniversityObjectives: Hwangryunhaedok-tang (HH) is a representative heat clearing multiherb prescription. We evaluated the protective effect of HH against heat stress exposure in mice.Methods: Six weeks old ICR mice were used for this study. After 43 ℃ heat stress for 15 minutes, we evaluated the changes of motor activity and c-fos expression level to determine the proper heat stress and evaluation time. The subjects were divided into 4 groups (1. control group, 2. heat stress group w ith normal saline, 3. heat stress group with administration of 100 mg/kg, and 4. h eat stress group with administration of 1000 mg/kg). After oral administration of HH once a day for 3 days, 2, 3, 4 groups were exposed to 43 ℃ heat stress for 15 minutes. Then, we evaluated the motor activity for 120 minutes and analyzed the c-fos expre ssion using western blot. We investigated the effect of HH and its ingredients on c -fos expressions after heat stress. The mice were administrated HH, Scutellariae Radix, Coptidis Rhizoma , Phellodendri Cortex , and Gardeniae Fructus for 3 days. After one hour of last treatment, the mice were exposed to heat stress at 43 ℃ for 15 min. After two hours, the hypothalamus was dissociated and lysed to measure c-fos expression.Results: After oral administration of HH for 3 days, motor activity was recovered insignificantly. 100 mg/kg HH treatment reduced the c-fos expre ssion after heat stress but insignificantly. Among the ingredients of HH, Coptidis Rhizoma and Scutellariae Radix treatment groups significantly reduced the c-fos after stress.Conclusions: These results show that Hwangryunhaedok-tang may be effective to reduce the heat stress response.Key Words: Heat Stress, Hwangryunhaedok-tang , Motor Activity, C-fos, Coptidis Rhizoma, Scutellariae Radix