Abstract
The quantitative determination of flavonoids in the above-ground part of greater knapweed (Centaurea scabiosa L.) has been carried out by differential spectrophotometry in combination with a modified method of forming flavonoid complexes with aluminium chloride in hydrochloric acid. Depending on the site of plant collection, the quantitative content of flavonoids in the aboveground part of greater knapweed varied within 1.3 - 2.0% (as recalculated for rutin). The random error of measurement did not exceed 3% at а confidence probability of 0.95. The systematical error of the proposed method did not exceed the error of determination. The dynamics of accumulation of flavonoids in above-ground part of greater knapweed during vegetative period in 2006 has been traced for the plants growing in the Tomsk and Kemerovo regions. The maximum content of flavonoids (1.70 ± 0.04% recalculated for rutin) was observed in the stage of full blossom, which is therefore the optimum period for collecting the above-ground part of plants for the maximum yield of flavonoids. The maximum amount of flavonoids was accumulated in leaves (1.96 ± 0.04 %), which is somewhat greater than the content of flavonoids in the entire above-ground part of greater knapweed (1.70 ± 0.04%).
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